At a glance
- Location
- Rockville · Axle Informatics LLC
- Salary
- USD 110,000-120,000 · Base salary
About the role
- Axle is seeking a Scientist to join our vibrant team at the National Institutes of Health (NIH) supporting the National Eye Institute (NEI) located in Bethesda, MD.
- The Scientist will independently support NEI by advancing research objectives through experimental design and execution.
- Working with the principal investigator, the Scientist will identify appropriate methods and protocols, develop and optimize experimental procedures, troubleshoot methodological issues, and implement corrective actions as needed.
Responsibilities
- Conduct animal studies including mouse handling, colony maintenance, tissue dissection, and sample collection
- Perform immunology and molecular biology assays, including flow cytometry, tissue culture, immunohistochemistry (IHC), quantitative polymerase chain reaction (qPCR), ATAC-seq, RNA-seq, and ChIP-seq
- Perform the inducible autoimmunity mouse disease model, experimental autoimmune encephalomyelitis (EAE)
- Culture and differentiate primary T cells for adoptive transfer
- Support the planning and execution of research experiments in the context of the laboratory's overall research program
- Independently direct and conduct high-priority special research projects, with special emphasis on developing novel research treatments
- Operate, calibrate, maintain, and troubleshoot laboratory instruments, equipment, and software
- Train staff on the proper use of laboratory instruments, equipment, and software
- Evaluate, analyze, and interpret research data, and communicate results and methods in technical reports, presentations, and manuscripts
- Maintain a detailed, up-to-date electronic laboratory notebook documenting all experiments
Requirements
- Master's degree
- Biosafety Level 2 (BSL-2) certification
Benefits
- 100% Medical, Dental & Vision Coverage for Employees
- Paid Time Off and Paid Holidays
- 401K match up to 5%
- Employee Referral Bonus
- Flexible Spending Accounts: Healthcare (FSA)
- Parking Reimbursement Account (PRK)
- Dependent Care Assistant Program (DCAP)
- Transportation Reimbursement Account (TRN)
Skills
- Animal Handling (mouse)
- Animal Behavior
- Tissue Dissection
- Genotyping
- Pipetting
- Polymerase Chain Reaction (PCR)
- Gel Electrophoresis
- Library Preparation
- Nucleic Acid Extraction
- Cell Culture And Primary Cell Culture
- Data Analysis And Data Presentation
- Standard Operating Procedure (SOP) Writing
Show all 15 skills
- Equipment Maintenance
- Inventory Management And Ordering Supplies
- PowerPoint
